Title: Associate Professor
College: Public Health and Health Professions
Department: Environmental and Global Health
Curriculum vitae: PDF
Research Interests: Avian influenza virus H5N1 and other influenza viruses (human and animal): detection, isolation, and genetic analysis; Live agent bioaerosol inhalation studies; Cultivation of alga and extraction of oil from alga; Production of biodiesel from algal oils; Respiratory viruses including hantaviruses and Sars coronavirus; Polyomavirus (BKV, JCV, LPV, PyV, SV40) regulatory region structure and function; Molecular and classical diagnostic virology (human and animal); Rodent- and lagomorph-pathogen serology and diagnostic virology; Paramyxovirus detection, isolation, and genetic analysis, and immunogenicity of Canine distemper virus; Development of human and animal virus detection, isolation, and identification methodologies; Determination of whole genomic sequences of various DNA and RNA viruses; Diagnostic (clinical) microbiology (bacteriology, mycology, parasitology, and virology); Reverse genetics of RNA viruses: expression of viral genes and virus production; West Nile virus: detection, isolation, and molecular characterization.
Hobbies: Cooking and dining, playing soccer, scuba diving, hunting, wildlife viewing and attending cultural events
Dr. John Lednicky has broad training and experience in microbiology and molecular biology, and performs both basic and applied research. He has worked with various bacteria, fungi, and viruses, some of which are select agents, in a variety of settings that include A/BSL3 laboratories. At the University of Florida, he is establishing an aerobiology laboratory for BSL2 and BSL3 work using a nose-only inhalation exposure system to study the effects of respiring aerosolized pathogens. Work in the aerobiology laboratory includes mechanisms of pathology by respiratory pathogens, assessments of inhalation threats posed by airborne pathogens, vaccine efficacy/safety studies, and the refinement of air-sampling technologies for the detection of airborne pathogens.
At his previous job at MRIGlobal, first as Principal Scientist, then as Senior Advisor, he managed and provided technical oversight and guidance for programs involving molecular biology, virology, and microbiology; served as a corporate expert in the area of virology and molecular biology; developed and directed research programs; wrote and reviewed research proposals, technical reports, and presentations; and managed technical aspects, schedules, and budgets of programs. While there, he established a ferret model for intranasal and aerosol studies with H5N1 and other influenza viruses.
His other work includes DNA and RNA virus discovery/characterization, and the reverse genetics of RNA viruses. He has isolated and/or detected metapneumoviruses, unusual reassortant influenza A viruses, neurotropic Canine distemper viruses, an arenavirus, and human and murine coronaviruses. Recent work includes the genetic characterization of a unique rhinovirus C, and an obscure human polyomavirus. The Lednicky laboratory also engineers cells that express specific viral receptors; these cells facilitate the isolation and propagation of the viruses that bind the receptors. Finally, the laboratory also evaluates antivirals (synthetic and natural) in-vitro and in animal models.
Environmental and Global Health
College of Public Health and Health Professions
101 S. Newel Drive (HPNP Bldg.) Rm 2146
Gainesville, Florida 32610-0188
Office phone: 352-273-9204
Fax: (352) 273-6070
Emerging Pathogens Institute
P.O. Box 100009
Gainesville, Florida 32610-0009
Voice: (352) 273-7526
Fax: (352) 273-9430